This work was developed with the objective of monitoring the occurrence of glyphosate-resistant biotypes of sourgrass (D. insularis) in Machado, Alpinópolis, Serrania and Divisa Nova, in the south region of Minas Gerais, Brazil. In each area, seeds of at least 20 plants were collected in full physiological maturity stage. For operational reasons, the analysis of different biotypes was divided into two timings, the first held in the second half of 2013 and the second in the first half of 2014. […]