Revista Brasileira De Herbicidas 2005; 4(3): 18-34

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GLYPHOSATE AND GLUFOSINATE AS SELECTIVE AGENTS FOR GENETIC TRANSFORMATION OF YELLOW PASSION FRUIT (Passiflora edulis f. flavicarpa Deg.)

DOI: 10.7824/rbh.v4i3.34

The yellow passion fruit is one of the most interesting economic species of Passifloraceae family. The biotechnology tools are useful to solve some problems that affect the productive potencial of this specie, like fungal and bacterial diseases, and the creation of more productivity varieties. In this context an experiment of yellow passion fruit was corried out to evaluate the glyphosate and gluphosinato herbicides as selective agents for genetic transformation. The culture medium used was Murashige & Skoog (1962) supplemented with B5 vitamin and organic complex, 30 g.L-1 sucrose, 100 mg.L-1 inositol, 1 mg.L-1 6– benzilaminopurina, solidified with 2,6 g.L-1 Phytagel. The experimental design was entire occasional and the bud regeneration and rooting formation experiments were evaluated. The glyphosate concentrations used in experiments of bud regeneration were: 0; 0,2; 0,4; 0,6; 0,8; 1,0 mg.L-1, for rooting experiments: 0; 0,05; 0,1; 0,15; 0,20; 0,30; 0,40 mg.L-1. The glufosinate treatments for bud regeneration were: 0,0; 0,2; 0,4; 0,6; 0,8 mg.L-1 and for rooting experiments: 0,0; 0,1; 0,15; 0,20; 0,25 mg.L-1. Number of explant with shoots and number of shoots per explant were evaluated in the experiment of caulogenesis. For rooting evaluation: number of rooted apexes, root size, primary root number and root fresh weight. Both herbicides glyphosate and glufosinate were efficient in the inhibition of the bud regeneration to a low concentration (0,785 mg.L-1 e 0,768mg.L-1 respectively), as well as in the inhibition of rooting (0,404 mg.L-1 e 0,249 mg.L-1 respectively). That allows the recommendation of those herbicides as selective agents in tissue culture of passion fruit for works of genetic transfer.