Revista Brasileira De Herbicidas 2000; 1(3): 261-265
Identification of glyphosate-tolerant soybean plants through germination test
DOI: 10.7824/rbh.v1i3.344
Since DNA tests are relatively expensive and time-consuming for the detection of the presence of the gene in plant materiais confering glyphosate tolerance to soybean, an experiment was carried out with the objective of developing a rapid and simple laboratory test for identifying glyphosate-tolerant soybean seeds, through standard seed germination tests. Seeds from six susceptible and twelve glyphosate-tolerant genotypes were used. The treatments consisted of cotiledonar/foliar spray of a glyphosate solution at the concentration of 8960 mg/l with 100 Ilha spray volume (equivalent to 2 g/ha) on the fifth day from the beginning of the germination test and soaking of the germination paper with glyphosate solutions at the concentrations of 0, 280, 560, 1120,2240,4480,6720, and 8960 mgll. The experimental units consisted of roIled germtest paper containing 50 seeds, replicated 8 times, except for the foliar spray treatment where 25 seeds were lined on the first third ofthe germtest paper. The germtest paper was weighed and the glyphosate solutions were added according to the treatments, in volume equivalent to three times the paper weight. Percentage of normal, abnormal and dead seedlings was assessed for eight days, by which time germination had ceased. This research demonstrated that it is possible to detect the presence of glyphosate-tolerant soybean seeds by the lab germination test using glyphosate at the rate of280 mg!liter ofthe imbibition water.
119